" />

Latest Cover

Online Office

Contact Us

Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
Tel:+86-28-85410485
Fax:+86-28-85410485
Email:[email protected] & [email protected]
Your Position :Home->Past Journals Catalog->2020 Vol.39 No.1

Development of Polymorphic Microsatellite Markers for Ailuropoda melanoleuca Based on RNA-Sequencing
Author of the article:TU Hongmei, ZHOU Chuang, WANG Guannan, CHENG Meiling, YUE Bisong, MENG Yang*
Author's Workplace:Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China
Key Words:Ailuropoda melanoleuca; genome; transcriptome; polymorphic; microsatellite
Abstract:Based on the published genomes of giant pandas (Ailuropoda melanoleuca), transcriptome of 6 giant pandas were sequenced in this study to screen the polymorphic microsatellite loci and analyze their composition and characteristics. The results showed that a total of 326 polymorphic microsatellite loci were obtained, of which the dinucleotide polymorphism microsatellites accounted for a maximum of 69.93% (228 in total). The proportions of tri-, tetra-, penta-, and hexanucleotide microsatellite loci were 9.51%, 14.11%, 5.21%, and 1.22%, respectively. According to the 2 indicators, deletion rate and standard deviation, and the length of the sequence, 20 dinucleotide microsatellite loci were selected and verified in 25 giant panda individuals. Further analyses showed that the number of alleles at different loci ranged from 2 to 8, with an average value of 3.70. The observed heterozygosity and the expected heterozygosity ranged from 0 to 1.000 and 0.280 to 0.784 with the average values 0.472 and 0.532, respectively. After Bonferroni correction, it was confirmed that the 4 loci significantly deviated from the Hardy-Weinberg Equilibrium, and no significant linkage disequilibrium was observed at all loci (P>0.01). The polymorphic information content (PIC) of 20 loci ranged from 0.246 to 0.734, with 9 highly polymorphic loci (PIC>0.50), and 11 loci were moderately polymorphic (0.25 < PIC < 0.50). The microsatellite loci screened in this study can help to assess the genetic diversity and population structure of giant pandas, to develop effective conservation and management strategies, and provide resources for the subsequent development of more excellent microsatellite loci for the genetic study of giant panda population.
2020,39(1): 15-22 收稿日期:2019-08-07
分類號:Q959.8;Q347
基金項目:國家科技攻關項目(2016YFC0503200);國家自然科學基金項目(31702032)
作者簡介:涂洪梅(1995-),女,碩士研究生,研究方向:分子生態學,E-mail:[email protected]
*通信作者:孟楊,E-mail:[email protected]
參考文獻:
李薇, 李久煊, 荊慧芳, 等. 2017. 基于高通量測序的達氏鱘微衛星標記篩選[J]. 動物學雜志, 52(3):449-457.
李午佼, 李玉芝, 杜聯明, 等. 2014. 大熊貓和北極熊基因組微衛星分布特征比較分析[J]. 四川動物, 33(6):874-878.
喬麥菊, 冉江洪, 張和民, 等. 2019. 微衛星標記在大熊貓研究中的應用進展[J]. 獸類學報, 39(1):103-110.
青菁. 2016. 四川省大熊貓棲息地破碎化現狀研究及廊道規劃[D]. 南充:西華師范大學.
宋琪, 劉金龍, 郭憲光, 等. 2019. 基于Roche 454 GS FLX高通量測序的葉城沙蜥基因組微衛星特征分析[J]. 四川動物, 38(1):62-67.
唐小平, 賈建生, 王志臣, 等. 2015. 全國第四次大熊貓調查方案設計及主要結果分析[J]. 林業資源管理, (1):11-16.
修云芳, 李碧春, 陳玉村, 等. 2015. 應用微衛星分型進行小熊貓親子鑒定(英文)[J]. 獸類學報, 35(1):55-64.
岳華梅, 翟晴, 宋明月, 等. 2016. 基于轉錄組測序的興國紅鯉微衛星標記篩選[J]. 淡水漁業, 46(1):24-28.
張正義, 邢秀梅, 胡鵬飛, 等. 2017. 微衛星標記在動物遺傳多樣性分析的研究進展[J]. 經濟動物學報, 21(3):164-168.
Botstein D, White RL, Skolnick M, et al. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J]. American Journal of Human Genetics, 32:314-324.
Du L, Zhang C, Liu Q, et al. 2017. Krait:an ultrafast tool for genome-wide survey of microsatellites and primer design[J]. Bioinformatics, 34(4):681-683.
Du LM, Li YZ, Zhang XY, et al. 2013. MSDB:a user-friendly program for reporting distribution and building databases of microsatellites from genome sequences[J]. Journal of Heredity, 104(1):154-157.
Haas BJ, Papanicolaou A, Yassour M, et al. 2013. De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis[J]. Nature Protocols, 8(8):1494-1512.
He W, Lin L, Shen FJ, et al. 2008. Genetic diversities of the giant panda (Ailuropoda melanoleuca) in Wanglang and Baoxing Nature Reserve[J]. Conservation Genetics, 9:1541-1546.
Hu Y, Zhan X, Qi D, et al. 2010. Spatial genetic structure and dispersal of giant pandas on a mountain-range scale[J]. Conservation Genetics, 11(6):2145-2155.
Huang J, Li YZ, Du LM, et al. 2015. Genome-wide survey and analysis of microsatellites in giant panda (Ailuropoda melanoleuca), with a focus on the applications of a novel microsatellite marker system[J]. BMC Genomics, 16(1):61. DOI:10.1186/s12864-015-1268-z.
Jurka J, Pethiyagoda C. 1995. Simple repetitive DNA sequences from primates:compilation and analysis[J]. Journal of Molecular Evolution, 40(2):120-126.
Li WZ, Godzik A. 2006. Cd-hit:a fast program for clustering and comparing large sets of protein or nucleotide sequences[J]. Bioinformatics, 22(13):1658-1659.
Li YZ, Xu X, Shen FJ, et al. 2010. Development of new tetranucleotide microsatellite loci and assessment of genetic variation of giant panda in two largest giant panda captive breeding populations[J]. Journal of Zoology (London), 282(1):39-46.
Liu HG, Yang Z, Tang HY, et al. 2017. Microsatellite development and characterization for Saurogobio dabryi Bleeker, 1871 in a Yangtze river-connected lake, China[J]. Journal of Genetics, 96:1-4.
Lu Z, Johnson WE, Menotti-Raymond M, et al. 2001. Patterns of genetic diversity in remaining giant panda populations[J]. Conservation Biology, 15(6):1596-1607.
Ma HY, Yue YS, Lu Y. 2004. Microsatellite DNA molecular marker and its application in animal breeding and genetics[J]. Journal of Animal Science & Veterinary Medicine, 23(5):16-19.
Marshall TC, Slate JB, Kruuk LE, et al. 1998. Statistical confidence for likelihood-based paternity inference in natural populations[J]. Molecular Ecology, 7(5):639-655.
Martin M. 2015. Cutadapt removes adapter sequences from high-throughput sequencing reads[J/OL]. EMBnet.jour-nal, 17(1):10-12[2019-05-05]. https://journal.embnet.org/index.php/embnetjournal/article/view/200/479. DOI:10.14806/ej.17.1.200.
Nagy S. 2012. PICcalc:an online program to calculate polymorphic information content for molecular genetic studies[J]. Biochemical Genetics, 50(9-10):670-672.
Qi WH, Jiang XM, Du LM, et al. 2015. Genome-wide survey and analysis of microsatellite sequences in bovid species[J/OL]. PLoS ONE, 10:e0133667[2019-05-05]. https://doi.org/10.1371/journal.pone.0133667.
Raymond M, Rousset F. 1995. GENEPOP (version 1.2):population genetics software for exact tests and ecumenicism[J]. Heredity, 86(3):248-249.
Ritchie H, Jamieson AJ, Piertney SB. 2016. Isolation and characterisation of microsatellite DNA markers in the deep-sea amphipod Paralicella tenuipes by Illumina Miseq sequencing[J]. Journal of Heredity, 107(4):367-371.
Selkoe KA, Toonen RJ. 2006. Microsatellites for ecologists:a practical guide to using and evaluating microsatellite markers[J]. Ecology Letters, 9(5):615-629.
Senanan W, Kapuscunski AR, Na-Nakorn U, et al. 2004. Genetic impacts of hybrid catfish faming (Clarias macrocephalus×C. gariepinus) on native catfish populations in central Thailand[J]. Aquaculture, 235(1-4):167-184.
Shen FJ, Watts P, He ZZ, et al. 2005. Enrichment of giant panda microsatellite markers using dynal magnet beads[J]. Acta Genetica Sinica, 32(5):457-462.
Shete S, Tiwari H, Elston RC. 2000. On estimating the heterozygosity and polymorphic information content value[J]. Theoretical Population Biology, 57(3):265-271.
Tian CX, Liang XF, Yang M, et al. 2014. New microsatellite loci for the mandarin fish Siniperca chuatsi and their application in population genetic analysis[J]. Genetics and Molecular Research, 13(1):546-558.
Wang D, Hu Y, Ma T, et al. 2016. Noninvasive genetics provides insights into the population size and genetic diversity of an Amur tiger population in China[J]. Integrative Zoology, 11(1):16-24.
Wei F, Hu Y, Zhu L, et al. 2012. Black and white and read all over:the past, present and future of giant panda genetics[J]. Molecular Ecology, 21(23):5660-5674.
Wu H, Zhan XJ, Zhang ZJ, et al. 2008. Thirty-three microsatellite loci for noninvasive genetic studies of the giant panda (Ailuropoda melanoleuca)[J]. Conservation Genetics, 10(3):649-652.
Xia EH, Yao QY, Zhang HB, et al. 2016. CandiSSR:an efficient pipeline used for identifying candidate polymorphic SSRs based on multiple assembled sequences[J/OL]. Frontiers in Plant Science, 6:1171[2019-05-05]. https://doi.org/10.3389/fpls.2015.01171.
Xiao TQ, Lu CY, Xu YL, et al. 2015. Screening of SSR markers as sociated with scale cover pattern and mapped to a genetic linkage map of common carp (Cyprinus carpio L.)[J]. Journal of Applied Genetics, 56(2):261-269.
Xu Y, Hu Z, Wang C, et al. 2016. Characterization of perfect microsatellite based on genome-wide and chromosome level in rhesus monkey (Macaca mulatta)[J]. Gene, 592:269-275.
Zheng XF, Pan C, Diao Y, et al. 2013. Development of microsatellite markers by transcriptome sequencing in two species of Amorphophallus (Araceae)[J/OL]. BMC Genomics, 14(1):490[2019-05-05]. https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-14-490.
CopyRight©2020 Editorial Office of Sichuan Journal of Zoology
疯狂飞艇是官方彩票吗 一分彩玩法图片 快3一分钟玩法漏洞 七星彩开奖结果走势图 山东11选5一定牛分布图 股票分析报告2020 湖北快3组合走势图 谁有极速赛车技巧 福建体彩11选5真准网 最好的免费股票分析软件 内蒙古十一选五遗漏数据 福彩17500cm乐彩网 喜乐彩中奖查询 河南十一选五开奖时间 湖北十一选五遗漏一定牛 凤凰彩票app下载 七星彩定胆杀号精准科360